AMGEN-Bruce Wallace Lab
In this lab, students use recombinant DNA technology to create plasmids containing the red fluorescent protein, originally derived from a sea anemone species. After transforming bacteria with the plasmids and growing on plates to check for transformation, the protein is purified using column chromatography. Students also perform the polymerase chain reaction (PCR) using their own cheek epithelial cells.
For more information about this series of labs and to download the curriculum materials, including the Teacher Guide and PowerPoint presentations, visit the Amgen-Bruce Wallace Program website. Please note: to view the Teacher Guide downloaded from the website, you must obtain a password from your partnership coordinator. However, if you are a BABEC authenticated teacher, you can sign in to download the guide directly here.
These labs were developed by Dr. Marty Ikkanda at Pierce College.
Download the Amgen-Bruce Wallace Laboratory Guide. Note: The Teacher Guide is located at the very bottom of the page. You must be signed in as an authenticated teacher to view the Teacher Guide .