AMGEN/ Bruce Wallace Lab
In this lab, students use recombinant DNA technology to create plasmids containing the red fluorescent protein, originally derived from a sea anemone species. After transforming bacteria with the plasmids and growing on plates to check for transformation, the protein is purified using column chromatography. Students also perform the polymerase chain reaction (PCR) using their own cheek epithelial cells.
(This lab was created by Dr. Marty Ikkanda at Pierce College. The student guide is available for download here.)