AMGEN-Bruce Wallace Lab
In this lab, students learn about the process of engineering a recombinant plasmid to produce purified protein. There are two series of labs available to study red fluorescent protein (RFP). In the basic series, students can study a recombinant RFP plasmid pARA-R in restriction digest, gel electrophoresis, and transformation labs. In the genetic enginerring series, students use recombinant DNA technology to create a plasmid containing both the red fluorescent protein and transform bacteria with the pARA-R plasmid they made. After transforming bacteria with pARA-R in either series, the protein may be purified using column chromatography or batch purification.
For more information about this series of labs and to download the curriculum materials, including the Teacher Guide and PowerPoint presentations, visit the Amgen-Bruce Wallace Program website. Please note: to view the Teacher Guide downloaded from the website, you must obtain a password from your partnership coordinator. However, if you are a BABEC authenticated teacher, you can download the guide directly here.
These labs were developed by Dr. Marty Ikkanda at Pierce College.
Each module in this series is available both as student and teacher versions. To view the teacher guide version in the "Comments" at the bottom of the page, you must be logged in as an authenticated teacher. If you do not know what this means, or cannot view the files below, please contact us.
Amgen-Bruce Wallace Student Guide
- Amgen-Bruce Wallace Student Guide (English) (9.18 MB)
- Amgen-Bruce Wallace Student Guide (Spanish) (3.72 MB)
- Basic restriction digest (Lab 2a)
- Basic gel electrophoresis (Lab 4a)
- Basic bacterial transformation (Lab 5a)
- Genetic Engineering series (Labs 2, 3, 4, and 5)
- Protein purification by HIC column chromatography (Labs 6 and 7)
Amgen-Bruce Wallace Nickel Affinity Protein Purification
- A-BW Nickel Affinity Protein Purification Protocol (160 KB)
- A-BW Nickel Affinity Protein Purification Worksheet (62 KB)