Alu PV92 PCR Lab
"Ah, Lou! There Are Differences Between Us!"
This unit introduces students to PCR, issues of identity and issues of privacy. Students isolate their cheek cell DNA from a saline mouthwash. They amplify their Alu PV92 locus by the polymerase chain reaction procedure, using pre-made master and primer mixes. Maintaining sample privacy, students electrophorese their reactions, stain and photograph their gels, and analyze their Alu PV92 genotype. Anonymously, data are entered into the Allele Server database at Cold Spring Harbor's DNA Learning Center. Computer follow-up activities include determining whether the class is in Hardy-Weinberg Equilibrium and plotting the "+" allele frequencies on a world map. Students compare their demographics to a map of global Alu distribution and brainstorm explanations for the original insertion of this particular Alu element in the human genome. This lab is recommended as a first PCR lab for high school students.
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