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D1S80 LabUsing the VNTR Locus D1S80 for Human Identification
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This
laboratory exercise is currently under development. Students will amplify
their own cheek cell DNA from a saline mouthwash and then PCR amplify their
D1S80 locus. Following PCR, students will electrophorese their PCR
products on TBE polyacrylamide gels. This allows for better separation of
the D1S80 alleles, which differ by 16 bp, and exposes students to another method
of electrophoresis. After staining and photographing their gels, students
will be ready for analysis. First, students will use a 50 bp DNA ladder of
molecular weight markers to generate a standard curve. They will measure
the distance each band in the marker lane migrated from the well and will then
calculate the log of that distance. Using a graphing calculator, students
will plot their "X" values (the log of the distance each band
migrated) and their "Y" values (the corresponding size of each band in
the 50 bp marker). After measuring the distance their allelic band(s)
traveled, students will use linear regression to determine their allele size(s). |
Copyright 2004 Bay Area Biotechnology Education Consortium |
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D1S80 LabUsing the VNTR Locus D1S80 for Human Identification
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Copyright 2004 Bay Area Biotechnology Education Consortium |