PCR Optimization: An Advanced Short Course For Teachers
(February 8th, 2003)
Workshop Description
BABEC and Applied Biosystems present this workshop on Saturday, February 8th,
2003, featuring calculations of two titration experiments in optimizing the PCR
reaction for Acetylcholinesterase(abbreviated AChE) gene segment. Agenda and
directions are below.
 Agenda
9:00 AM Welcome and Breakfast
9:15 Introductions
9:30 Introduction to PCR Optimization
9:45 Calculations
10:30 Set up PCR reactions
10:45 BREAK
11:00 Components of PCR
11:30 Make and pour gels
12:00 PM LUNCH
1:00 Electrophoresis
1:30 Lambda lab as precursor to Optimization
2:00 BREAK
2:15 Optimization overview
2:30 Stain and photograph gels
3:00 Analyze results
3:30 Evaluation and closure
4:00 Class dismissed
Location
John O'Connell High School
2355 Folsom Street
San Francisco, CA 94110
Room 321 (Enter through main entrance on Folsom Street.)
(click here for
a Yahoo map to John O'Connell High School)
If you have any questions, contact the Outreach Coordinator, Shalini Prasad,
at prasadsn@appliedbiosystems.com or (650)554-2990.
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